|Release date||7 November 2011 (Update)|
|Quest NPC?||One Piercing Note|
|Location||Abbey of St. Elspeth Citharede|
|Examine||A mad-eyed woman wrapped in rags.|
Not much is known about Anna prior to the events of the One Piercing Note quest, aside from the fact that she was good friends with Valerio and originally comes from Lumbridge. Valerio describes her as being a strong-minded woman and that nothing would change her mind once it was set, evidenced when he reveals that he was unable to change her mind when she decided to join the Abbey of St. Elspeth Citharede.
She described herself, to the other Sisters involved in the quest before her "death", as having a "sinful" life before joining the Abbey. Although what these "sins" were was never revealed, but what was revealed was that she seemed to have a love for dance. However, after joining the Abbey, she began to grow a strong disdain for anyone who would dance whilst inside the Abbey's walls
During the quest, the player is enrolled into investigating her murder. However, as the story-line progresses, the player finds out that Anna was not actually murdered, but was instead the murderer. She claimed to be possessed by the Spirit of Saint Elspeth, which led her to murder Sister Elena and Sister Catherina, as well as the applicant Isabella. Her motive for murdering them was because of their love of dancing, and considered them to be sinners as the Abbey did not permit dancing. It was hinted that during this time she was possessed by The Ripper, while convinced that she was instead possessed by the spirit of Saint Elspeth.
At the end of the quest, depending on what choice the player chose, Sister Anna either throws herself off the Bell Tower, is kicked off by the player or falls out while praying, thinking she can fly like an Icyene.
- In the bookcases of the Abbey are three books written by Anna: 'The Lives of the Seven Priestly Warriors', 'Saint Elspeth and Saint Zilyana ' and 'The Stillness of the Feet on the Path of Penitence'.
- Some players have drawn comparisons between Sister Anna and the Murder Mystery quest's Anna Sinclair and how she was the mastermind behind Lord Sinclair's death, while several other people were killed.